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Fig. 2 Neurohypophyseal vasculature is devoid of the BBB marker protein claudin 5. (A,B) Claudin 5 expression in hypophyseal vessels of 5 dpf larvae (A) or adult (B) was detected by whole-mount immunofluorescence staining using anti-claudin 5 antibody. Tg(kdrl:mCherry-caax) zebrafish. (C,D) Claudin 5 expression in brain vasculature was detected by whole-mount immunofluorescence staining using anti-claudin 5 antibody in 5 dpf larvae (C) or adult (D) Tg(kdrl:mCherry-caax) zebrafish. AMCtA, anterior (rostral) mesencephalic central artery; BCA, basal communicating artery; CrDI, cranial division of the internal carotid artery; HypA, hypophyseal artery; HypV, hypophyseal vein; PCS, posterior (caudal) communicating segment. Scale bars: 5 µm in A,C; 20 µm in B,D.