|
Fig. 3
Palmitic acid activates autophagy in zebrafish larvae. Zebrafish GFP-Lc3 transgenic embryos were treated with PA by bath immersion, and 24 h later, they were infected with SVCV for 24 h. The intensity of the GFP-Lc3 signal was measured in the body muscle mass (A) and liver (B). Numbers in the images represent the animals with the shown phenotype per the total number of animals analysed (C). The results are presented as the mean ± SEM of the analysed fish by treatment. Comparisons among groups were made using one-way ANOVA (multiple comparisons) followed by a Student-Newman-Keuls test. Significant differences are displayed as *** (0.0001 < p < 0.001), ** (0.001 < p < 0.01) or * (0.01 < p < 0.05).