ZFIN Image: Leon et al., 2020, Fig. 5

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Figure Caption

Fig. 5 The calcium-binding site is required for Gpr126 function in vivo.

a Surface conservation analysis (green, variable; purple, conserved) of CUB domain. The calcium-binding site is circled in magenta. D134 and F135 are indicated by arrows. b D134 and F135 were both mutated to alanines through homologous recombination of a 150 bp ssODN containing a 5 bp mutation (red nucleotides). c Genotyping assay for the gpr126^stl464 lesion. The 5 bp mutation introduces a BstUI restriction enzyme binding site. d 4 dpf wild-type larva compared to e 4 dpf gpr126^{stl464/stl464} larva with puffy ears (arrowheads). Scale bars (black) represent 100 μm. f 4 dpf wild-type larvae express mbp throughout the posterior lateral line nerve (PLLn, arrowhead), whereas g 4 dpf gpr126^{stl464/stl464} larva lack mbp expression along the PLLn (arrowhead). Scale bars (black) represent 100 μm. Asterisks indicate CNS.

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Acknowledgments

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