ZFIN Image: Li et al., 2019, Fig. 5

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Fig. 5

The FGF pathway did not play the most important role in the Sox19b regulation of NSCs. a Quantitative RT-PCR analysis is shown for fgf3, fgf8, and er81. Compared with the controls, the mRNA levels of fgf3 and er81 in Sox19b MO-injected embryos decreased significantly. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control. b Compared with embryos injected with Sox19b MO, the NTD appearance in zebrafish embryos was not rescued by the addition of fgf3 mRNA. c Quantitative RT-PCR analysis for Ngn1, Huc, and Islet1. Fgf3 mRNA could not change the increase in Ngn1, Huc, or lslet1 induced by Sox19b MO treatment. Data represent the mean of at least three independent experiments ± SD. *P < 0.05, **P < 0.01 versus control; ns, no significant difference

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Acknowledgments

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