ZFIN Image: Cheng et al., 2019, Figure 2

Image

Figure Caption

Figure 2 Knockdown of pparγ adversely affects development within the first 96 h.

Abundance of pparγ mRNA within whole zebrafish embryos from 0.75 to 96 hpf (A). Confirmation of knockdown by 24 hpf following injection of splice-blocking pparγ-MOs. Lanes 1–3: 6-hpf embryos injected with nc-MO; lanes 4–6: 6-hpf embryos injected with pparγ-MO; lanes 7–9: 24-hpf embryos injected with nc-MO; and lanes 10–12: 24-hpf embryos injected with pparγ-MO (B). Representative images of water-, nc-MO-, or pparγ-MO-injected embryos from 6 to 96 hpf before and after staining with Oil Red O (ORO) (C–FF). Arrows point to mild dorsoventral patterning defects (ventralization), whereas arrowheads point to cardiac edema and cardiac looping defects.

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Acknowledgments

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