Figure 3
Dkk1 Reduces Cell Adhesion
(A) Single-plane confocal imaging, dorsal view, of tail bud stage embryos (n = 6 of each condition) ubiquitously expressing EGFP-tagged myosin light chain (MyosinEGFP, green) stained with phalloidin (red) labeling filamentous actin. Scale bar, 20 μm.
(B) Single-plane confocal images of axial cells in
(C) Distribution of endogenous β-catenin (red) in
(D)
(E) Quantification of E-cadherin distribution across boundaries between neighboring axial (green) cells. Normalized intensity distributions across cell-cell boundaries show that Dkk1 expression reduces E-cadherin intensity at cell boundaries and broadens lateral distribution of E-cadherin intensity. Normalized intensity distributions were calculated for boundaries between six neighboring cells in five embryos for both control and Dkk1-expressing embryos. ∗∗∗p < 0.001. p value was calculated using the Kolmogorov-Smirnov test.
Reprinted from Developmental Cell, 51(6), Johansson, M., Giger, F.A., Fielding, T., Houart, C., Dkk1 Controls Cell-Cell Interaction through Regulation of Non-nuclear β-Catenin Pools, 775-786.e3, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell