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Fig. S7
Effects of eomesA, foxh1, and mixl1 misexpression on early developmental genes
(a-m) Zebrafish embryos ((a-l) shield to 75% epiboly or (m) 2 ss) as control or injected with mRNA for eomesA, foxh1, and mixl1 (e/f/m), with mRNA ISH for indicated candidate genes. Latera view left, animal view right for each condition. (a,b) The early LPM gene tmem88a that is weakly expressed during epiboly (a) becomes activated in patches upon e/f/m misexpression (b, arrowheads). (c-j) Expression of various gastrulation-stage marker genes: admp (c,d), sizzled (slightly increased expression ventral, indicated by arrowhead) (e,f), ved (slight increased expression ventral, arrow) (g,h), gata2a (slight ventral expansion) (i,j). (k-m) The somite-stage LPM gene hand2 does not become ectopically induced upon e/f/m misexpression (k-l), as tested with a functional mRNA ISH probe (shown for 2 ss in m). Indicated n-numbers represent the number of embryos analyzed. Scale bar (a) 250 μm