ZFIN Image: Nelson et al., 2019, FIGURE 3

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Figure Caption

FIGURE 3

Glucocorticoids inhibit the formation of trans-lesion glial bridges. (A–F) Whole-mount preparations (lateral view) of Tg(gfap:EGFP) transgenic zebrafish show immunostaining for EGFP (green) and DAPI staining (blue) from 24 h post sham injury (A,B), 24 h post SCI with no treatment (C,D) and 24 h post SCI+Dex (E,F). EGFP-positive ependymal glia were observed spanning the lesion site at 24 h post SCI in controls (C,D), and were absent with Dex (E,F). Boxed regions (A,C,E) are magnified by 175% (B,D,F, respectively). Scale bars, 50 μm (A is the same as C,E; B is the same as D,F). Representative images from 10 preparations per condition. (G) Counts of glial bridges at 24 h post SCI in and 24 h post SCI+Dex.

Acknowledgments

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