ZFIN Image: Hu et al., 2019, Fig. 6.

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Figure Caption

Fig. 6.

Increasing Hh signaling by Ptch1 knockdown might be beneficial for osteoblast development. Embryos were injected with Ptch1MO and Ptch1Mis at the 1–4 cell stage, at 6 dpf, larvae were collected for qPCR, western blot and Alizarin Red staining respectively. (A) QPCR showed osteoblast genes mRNA level. (B) Western blot results showed BMP2 and SP7 protein level. (C) Alizarin Red staining showed bone mineralization; blue rectangle, rib. (D) Col10a1a protein expression in cb5 (ceratobranchial 5, white circle) region was observed in Ptch1MO-injected group. *P<0.05, **P<0.01, ***P<0.001 versus the untreated groups (one-way ANOVA). The data were from three independent assays.

Acknowledgments

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