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Fig. 1
a–d Axon tracts of the forebrain in zebrafish microinjected with cep41 MOs or human CEP41 mRNAs as indicated in each panel. The axons immunostained with anti-acetylated α-Tub antibodies at 35 hpf were imaged by fluorescence microscopy. AC anterior commissures, POC post optic commissure, SOT supra optic tract, TPOC tract of the post optic commissure, TPC tract of the posterior commissure. Asterisks indicate reduced and disorganized axons. Scale bar is 100 µm. e Quantified data by counting zebrafish embryos with forebrain axonal defects. The severity of defective axons is distinguished as the numbers of malformed axonal tracts. Mild: 1–2 axonal defects. Severe: 3–5 axonal defects. f–i Axon tracts of the hindbrain in MOs or mRNA-injected zebrafish. r2, r4 and r6 are rhombomere 2, 4 and 6. Asterisks indicate defective axons and scale bar is 100 µm. j Quantified data by counting zebrafish embryos with hindbrain axonal defects. The severity of defected axons is distinguished as the numbers of abnormal axons. Mild: 1–6 axonal defects. Severe: 7–10 axonal defects
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