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Fig. 7
Failure of optic fissure fusion in ntn1a morphant zebrafish embryos. Expression levels (TPM, transcripts per million) of ntn1a candidate DEG showing temporal expression at 32,48 and 56 hours post fertilization (hpf) (A). (B) Wholemount and ocular morphology of 56 hpf wildtype (wt) and ntn1a morphant embryos, showing loss of ntn1a results in an ocular coloboma phenotype, scale bar 500 µm. (C) Histological analysis confirming loss of optic fissure fusion in ntn1a morphants. Scale bar 50 µm.
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