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Fig. S2

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ZDB-IMAGE-190613-10
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Figures for Bise et al., 2019
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Fig. S2

Components of the LIFR/GP130 pathway are transcriptionally upregulated after preconditioning. (a) Heat map representation of 20 differentially expressed genes at 1 dpt that contribute to the LIFR/gp130 pathway. Fold changes are represented in log2 scale of fold change (blue: log2 < 0; red: log2 > 0). (b) In-situ hybridization of ventricular sections demonstrates upregulation of several components of the LIFR/GP130 pathway at 1 dpt. n ≥ 3 hearts. (c) Detection of putative LIFR/gp130 ligands at 1 dpt by qRT-PCR. The normalized gene expression was calculated relative to β-actin. n ≥ 3 samples prepared of 10 ventricles. **P < 0.01 with student’s t-test. (d) In-situ hybridization of ventricular sections demonstrates upregulation of cntf transcription at the periphery of the ventricle, at 1 dpt. n ≥ 3 hearts.

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