|
Fig. 7
Stx1b morpholino oligos injection suppressed BBR activation on STX1B expression in the PTZ-model larvae. (A) Hybridization in situ results show that a change of the stx1b messenger RNA level in the larval (7 dpf) brain was induced by BBR with stx1b morpholino oligos injection in the PTZ-model zebrafish, as compared with in the wild-type, stx1b morpholino oligos injection, and PTZ plus stx1b morpholino oligos groups (n = 20). (B) Western blotting results indicated a change of the STX1B protein level similar to the change of the stx1b messenger RNA level under the same treatments (n = 3). &P < 0.05 vs. PTZ plus stx1b morpholino oligos model; 𝜃P < 0.05 and 𝜃𝜃P < 0.01 vs. stx1b morpholino oligos model.