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Fig. S1
(A) Excised larval intestines conrm statistically elevated numbers of L-plastin positive cells in germ-free intestines. No signicant dierence was observed in the intestines of Myd88 decient larvae. (mean ± s.e.m, n=2 biological replicates, 6-8 embryos per group), *p0,05; ***p0,001 by two-way ANOVA with Bonferroni correction for multiple comparisons. (B-C) Whole embryo assessment of overall leukocyte assessment by L-plastin immunostaining, (mean ± s.e.m, n=2 biological replicates, 6-8 embryos per group) Acquired by confocal microscopy at 5x magnication, no signicant dierences were observed by two-way ANOVA with Bonferroni correction for multiple comparisons. (D) Quantication of macrophages (magenta) and neutrophils (green) in the distal intestine of GF versus CONVD wt larvae of the Tg(Mpx:GFP/Mpeg:mCherryF) line)at 5 DPF, acquired by confocal microscopy at 20x magnication. The region of analysis is outlined in white lines, (mean ± s.e.m, n=3 biological replicates, 6-8 embryos per group) *p0,05; ***p0,001 by Student’s t-test. Scalebars, 200 μm in A; 500 μm in B-C; 100 μm in D.