ZFIN Image: Molinari et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

Mounting samples for visualization of Kupffer?s vesicle.

Schematic showing a 10 somite stage zebrafish embryo (A). Forceps can be used as depicted to separate the tail tip from the rest of the embryo. Excess yolk is then removed, and the tail tip mounted flat on to a microscope slide. (B) Bright field image overlaid with fluorescence image of flat-mounted tail tip. The white box depicts the location of Kupffer?s vesicle. Fluorescence from acetylated-? tubulin (red) can be seen within this region. (C) Confocal image of Kupffer?s vesicle, visualised using acetylated-? tubulin (red), aPKC (green) and DAPI (blue).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ F1000Res