ZFIN Image: Karra et al., 2018, Fig. 5

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Figure Caption

Fig. 5

Dependence of vegfaa-induced CM hyperplasia on Notch signaling. (A?D) In situ hybridization for notch1b and deltaC on cardiac sections from cmlc2:CreER;?act2:BS-vegfaa animals 14 dpi with vehicle or tamoxifen. Violet staining indicates expression. (E?H) Maximum-intensity projections of 7 dpf hearts from cmlc2:H2A-EGFP;?act2:BS-vegfaa fish with or without an additional cmlc2:CreER transgene. Animals were first treated with 4-HT, followed by DMSO (n = 9 for Cre^? animals and n = 10 for Cre⁺ animals) or DAPT (n = 8 for Cre^? animals and n = 9 for Cre⁺ animals). (I) Number of CMs at 7 dpf for treatment groups shown in E?H. Error bars indicate SD (*P < 0.05, **P < 0.01, and ***P < 0.001, Student?s t test). NS, not significant. [Scale bars: A and E, 100 ?m (also apply to B?D and F?H, respectively).]

Acknowledgments

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