ZFIN Image: López-Muñoz et al., 2018, Fig. 1

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Fig. 1

M. circinelloides R7B is highly pathogenic for zebrafish. Zebrafish were i.p. injected with 2.5 × 10⁵ or 10⁷ (high dose: HD) live or UV-killed sporangiospores of the R7B or NRRL strains. (A) The survival rates of infected zebrafish are shown. Each experimental group contained 20 zebrafish, and each experiment was performed in duplicate. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. (B) Gross morphological examinations of adult zebrafish infected with M. circinelloides R7B and NRRL were imaged at 16 hpi. (C) Histological analysis of M circinelloides-infected zebrafish. Transverse sections were obtained from zebrafish injected with 2.5 × 10⁵ R7B spores/fish or 10⁷ NRRL spores/fish at 16 hpi. Note that the mycelium (black arrows) can invade several organs and promotes a strong leukocyte infiltration (red arrows). L, liver; G, gut; M, muscle; O, ovary; T, testis. Scale bars 10 µm

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Acknowledgments

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