|
Fig. 9
GSK3 inhibition rescues 4-MU-induced larval tail regeneration and cell proliferation defects.
(A) Representative micrographs of larval tails that were amputated at 2 dpf and treated with 0.5% DMSO, 100 nM BIO, 150 μM 4-MU, or 150 μM 4-MU with 100 nM BIO for the next 24 hours. Caudal fins of 5-dpf (3-dpa) larvae are shown. Scale bar: 100 μm. (B) Caudal fin sizes at 5 dpf (3 dpa) for the indicated amputation and inhibitor treatment regimens (compound administration from 2 to 3 dpf). Data are the average caudal fin areas of 15 larvae ± s.e.m., normalized to the average fin size of uncut larvae treated with 0.5% DMSO. (C) Cell proliferation within the 4-dpf caudal fin in response to the indicated amputation and inhibitor treatment regimens. Data are the average number of pH3-positive cells in 30 larval tails ± s.e.m. (R1 + R2 regions; see Fig 9). *, P < 0.05; **, P < 0.01; ***, P < 0.001.