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Fig. 8
4-MU inhibits regenerative cell proliferation.
(A) Mitotic cells in the larval tail after the indicated amputation and 4-MU treatment regimens, as visualized with anti-pH3 immunostaining at 2 dpa (4 dpf). R1 and R2 demarcate distinct regions within the larval tail, with R1 corresponding to a highly proliferative 100-μm-wide zone associated with tail regeneration. (B) Quantification of pH3-positive cells in the R1 and R2 regions under the indicated treatment conditions. Data are the average number of pH3-positive cells in 30 larval tails ± s.e.m. **, P < 0.01.
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