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Fig. 7
Aqp0a is required for anterior suture stability. Fixed and permeabilized large adult zebrafish lenses were labeled for F-Actin with phalloidin (red) and nuclei with DAPI (blue). Maximum z-projections were generated from 150 μm z-stacks of the anterior pole (A) and 150 μm z-stacks of the posterior pole (D) revealing the anterior, and posterior sutures, respectively. Optical slices 46 μm from the anterior pole reveal detail of the anterior sutures, which are severely damaged in aqp0a−/− and double mutant lenses (B). High power images of peripheral fiber cells and epithelial cells from an optical slice taken 76 μm from the anterior pole reveal the presence of nuclei in fiber cells in mutant lenses compared to WT (C). The cell nuclei within fiber cells counted in the whole optical slice taken 76 μm for WT: 0, aqp0a−/−: 38, aqp0b−/−: 2, double mutants: 21.
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Full text @ Invest. Ophthalmol. Vis. Sci.