Fig. 2

Loss of Aqp0a and/or Aqp0b increases frequency of embryonic cataract. (A) Representive in vivo DIC images of clear and cataractous lenses from WT, aqp0a^−/−, aqp0b^−/− or double mutant embryos taken at 2, 3, and 4 dpf. Refractive defects at the cortex-nucleus interface at 4 dpf are indicated by white arrows. (B) Cataract frequency was quantified at 2, 3, and 4 dpf by binary readout. A statistical test of proportions was used to estimate the difference in incidence rate of cataracts between each pair of genotype groups. The Holm method was used to calculate adjusted P values that account for multiple testing. Statistically significant differences at 95% confidence are indicated by an asterisk. See Supplementary Figure S1 for full statistical analyses.