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Fig. S2
RNA in situ hybridization reveals islet2a expression in a subpopulation of SMNs.
(A-D) RNA in situ hybridization was performed using transgenic lines that express gfp in either dSMNs (tg(isl1:gfp); A, B) or vSMNs (tg(gata2:gfp); C, D). The red RNA in situ hybridization signal for islet2a is not detected in gfp+ dorsally-projecting SMNs at either 24 (A) or 48 (B) hpf. In contrast, islet2a RNA is detected in a subset of ventrally projecting SMNs (C, D). Scale bar in D, for A-D: 25 μm.
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