ZFIN Image: Klatt Shaw et al., 2018, Fig. 4

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Figure Caption

Fig. 4

RyR Function Is Required for Development of Shh-Dependent Neural Crest-Derived Neurons of the DRG and the ENS

(A–L) Lateral views of live 72 hpf Tg(isl2b:GFP) embryos with GFP-labeled Rohon-Beard neurons (arrow) and DRGs (arrowhead). Embryos were treated between 24 and 48 hpf with cyclopamine (cyc), azumolene (azum), ryanodine (ryan), N-acetyl cysteine (NAC), aldrithiol, thapsigargin (thaps), or tricaine at the indicated concentrations, or injected at the one-cell stage with ryr1a and ryr3 MOs. Arrowheads in (C and G) indicate the presence of small, faint DRGs.

(M) Quantification of embryos treated as in (A–L). DRGs present in somites 11–15 were counted. For each condition, the number of embryos analyzed is indicated. Comparisons are to control vehicle-treated embryos unless otherwise indicated. Data are represented as means ± SEM. ^∗p < 0.01; ^∗∗∗p < 0.0001; n.s., not significant.

(N–Q) Lateral views of live 78 hpf Tg(phox2bb:GFP) embryos with the ENS (arrow) labeled by GFP in control, cyclopamine-treated, ryr1a,3 morphant, or ryanodine-treated embryos. Black melanophores (pigment) are visible in each condition. Scale bar, 25 μm in (A).

See also Figure S4.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 45(4), Klatt Shaw, D., Gunther, D., Jurynec, M.J., Chagovetz, A.A., Ritchie, E., Grunwald, D.J., Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling, 512-525.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell