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Fig. 2
Isolation of Decision-Making Mutants from a Forward Genetic Screen
(A) Average startle biases of decision-making mutants, presenting the average bias of the “bottom 25%” of all tested larvae from heterozygous mutant carrier incrosses (n ⩾ 47 mutant larvae each), with the bottom 25% of representative wild-type larvae (Tüpfel long fin [TLF], indicated in blue; n = 28).
(B) Temporal projection over 40 ms post-stimulus (26 dB) of wild-type and wrong turn mutant 5-dpf larval responses. Percentage indicates average frequency observed (30 WT and 58 wrong turn larvae).
(C) Percentage of short- and long-latency responses using pectoral fins during the initial C-bend. n = 14 sibling (blue, 250 responses), and n = 14 wrong turn (red, 112 responses), Fisher’s exact test.
(D and E) Acoustic stimulus intensity versus average relative startle index (D) or average overall startle responsiveness (E) for wild-type (blue) and wrong turn mutants (red).
(F) Average relative startle bias of 5-dpf wrong turn and wild-type larvae following identical 26-dB stimuli at 20-s intervals (stimuli 1–10) and then 1–s intervals (stimuli 11–40).
(G) Zebrafish CaSR protein showing locations of the wrong turnp190 and CaSRp198 mutations, Signal Sequence (S, yellow), extracellular Venus Fly Trap domain (VFT, blue), cysteine-rich domain (CRD, pink), 7-pass transmembrane domain (7TMD, orange), C-terminal domain (CTD, green), 5 key residues of the ligand-binding pocket of the VFT hinge (light blue), and PKC phosphorylation residue (arrowhead).
Error bars indicate SEM. ∗∗p < 0.01; ∗∗∗∗p < 0.0001, Bonferroni-corrected t test. NS, not significant. See also Figures S3 and S4.