Fig. 2

Taxol and Nocodazole affect yolk cell microtubule organization and dynamics. (a) Live imaging of YCL labeled with EMTB-3GFP (green) and EB3-mCherry (white, tracking highlighted in red) in DMSO control and Taxol treated wild-type embryos at 60% epiboly. Time series projection of 4 consecutive time points (5.1 s in total). Scale bar 5 µm. (b-d) Microtubule growth rates (b), growth track velocity distribution (c), and average track speed (d) at 60% epiboly for DMSO control and three Taxol concentrations. Error bars - SEM. p *<0.05, **<0.005, Mann-Whitney test in d. (e) Microtubule growth track bending for DMSO control and Taxol treated embryos. (f) Sphere wild-type embryos anti-β-tubulin HRP/DAB immuno-histology. DMSO control, Taxol or Nocodazole treated embryos as indicated. Taxol: white arrows mark microtubule accumulation around MTOCs, black arrowheads mark denser YCL microtubule arrangement, black arrows mark irregular bundling of YCL microtubules upon Taxol treatment, white arrowheads mark small MT free patches. Nocodazole: black asterisk marks remaining MTOC and coherent YSN with emanating microtubules. Scale bar 100 µm. (g) Images of live YCL region of DMSO control and Nocodazole treated wild-type embryos at 40% epiboly. Scale bar 5 µm. (h-i) Track velocity distribution (h) and microtubule growth rates (i) at 40% epiboly in DMSO versus Nocodazole treated embryos. Error bars - SEM.