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Fig. 1
Zebrafish cyp21a2 expression is largely restricted to the interrenal gland. (a) Analysis of cyp21a2 expression during embryonic and early larval development by RT-PCR with b-actin1 as an internal standard (gel images inversed). A no-template control sample (NTC) served as negative control. The onset of detectable cyp21a2 expression is at 28 hpf, after which cyp21a2 continues to be expressed at all stages examined. (b) Images of wild-type larvae at different stages after WISH against cyp21a2. Left panels show lateral view, with head to the left; right panels show a dorsal view. No staining is seen in 24-hpf embryos. In 28-hpf embryos and 120-hpf larvae, strong staining is observed in the interrenal gland region, with some faint signal detected at 120 hpf in the head (arrows). Scale bar: 0.1 mm.