ZFIN Image: Cheng et al., 2017, Fig. 1

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Fig. 1

Mutation of lurap1 in zebrafish impairs CE movements. a Genomic organisation of lurap1 locus and TALEN-targeted regions. b Generation of an indel mutation in the second exon of lurap1 gene following a deletion of 7 nucleotides and an insertion of 12 nucleotides (green) between TALEN recognition sequences (yellow). Dashes are introduced in the WT sequence to optimise sequence alignment. c Frameshift and premature termination of translation in the lurap1 mutant transcript. d–g Live images of WT embryos at indicated stages, lateral view with animal pole or anterior region on the top. Arrowhead indicates the embryonic shield. The angle between the anterior end and posterior end, which reflects the extent of anteroposterior axis extension, is shown at 10 and 14 hpf. h, i Analysis by in situ hybridisation of dlx3 and papc expression patterns shows the extent of CE movements in WT embryos at 100% epiboly stage. j–m Phenotypes of MZlurap1 embryos at indicated stages show delayed embryonic shield formation and reduced anteroposterior axis elongation. n, o The expression patterns of dlx3 and papc in MZlurap1 embryos indicate defective CE movements. The neural plate is wider (horizontal line) and paraxial mesoderm is compressed (arrows). Scale bar: d–o 200 µm

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Acknowledgments

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