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Fig. 6
Defects in vascular endothelium and pancreas development of tcf7l2 exI/exI homozygous larvae. (A,B) Analysis of pancreatic islet and blood vessels inside and around the pancreas of tcf7l2 mutants and wild-type sib controls in Tg(fli1a:EGFP/ins:DsRed) background. Representative images were taken at 18 dpf by confocal microscopy at 20x magnification. (C) Graphic presentation of the integrated density of fluorescence in the red and green channel in tcf7l2 exI/exI mutant and wild-type sib controls at 18 dpf. The regions for the analysis of integrated density of fluorescence are indicated by white boxes. Data were obtained using Fiji software. (D) Analysis and graphic presentation of vessels diameter; the mutant is characterized by decreased vessel diameter. Data were obtained from five individuals per genotype. All reference to phenotypes was confirmed by genotyping; *p < 0.05.