ZFIN Image: Wu et al., 2017, Fig. S6

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Figure Caption

Fig. S6

Overexpression of cpn1 causes circulation defects and pericardial edema. (A-C) Injection of cpn1 mRNA into Tg(fli:eGFP^y1 ; gata1:dsRed) embryos causes circulation defect at ISV-DLAV at 48hpf. Quantification data showed overexpressing cpn1 embryos with 90% defects in circulation compared to control (n=10 for both conditions). (D-F) At 72hpf, overexpresion of cpn1 shows pericardial edema (arrow in E). Quantification of percentage of pericardial edema showed ~70 % increase in cpn1 mRNA injected embryos compared to control (n=33 in control and n=28 in cpn1 mRNA injection) at 72hpf. Scale bars are 100 μm for A, B and 200 μm for D, E.

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