ZFIN Image: Lu et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

Generation of miR-142a knock out zebrafish using CRISPR/Cas9.

(a) Schematic diagram showing the CRISPR/Cas9 target sites 1 and 2 in the miR-142a gene. (b) Genotyping showed different lengths of PCR products from 142C+/+ and 142^C-/- generated by CRISPR Cas9. (c) A 986 base-pair deletion caused by CRISPR/Cas9 in the miR-142a locus showed failure to form mature miR-142a-5p and miR-142a-3p. (d) Predicted RNA secondary structure of wide type and mutated pri-miR-142a by CRISPR/Cas9. (e) MiR-142a-3p was absent in 142^C-/- embryos at 4 dpf by WISH. Red dashed circles mark the expression of miR-142a-3p in the thymus and red arrows mark the expression of miR-142a-3p in the CHT.

Acknowledgments

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