ZFIN Image: Halluin et al., 2016, Fig. 7

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Figure Caption

Fig. 7

Autonomous reception of Hedgehog signals is required for habenular neurogenesis.

Confocal sections through the epithalamus of wild type host embryos containing transplanted cells from wild type (A) or smo^hi229 donors (B). Donors carried the Tg(huC:GFP), which appears in white, and transplanted cells appear in red; red cells that are not white in B are epiphysial photoreceptors (white arrow). Host embryos were immunolabelled with an anti-HuC/D antibody (in blue) that highlights the habenular neurons (Hb) and projection neurons in the epiphysis (ep). Embryos are viewed dorsally with anterior up. A histogram indicates the number of embryos incorporating cells from wild type or smo^hi229 mutant donors into either the habenulae or epiphysis (C). Unlike cells transplanted from wild type donors (white arrowheads in A), those from homozygous smo^hi229 mutants are systematically excluded from becoming habenular neurons; transplanted cells from either donor can become epiphysial projection neurons.

Acknowledgments

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