ZFIN Image: Dash et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Insulin-positive cells are increased in the intrapancreatic duct (IPD) of sept7b TBMO and DAPT -treated larvae. (A-D) Immunostaining of Tg(ptf1a:GFP) zebrafish larva co-treated with sept7b TBMO and 20 μM DAPT with antibodies against insulin shows cells positive for insulin (arrowheads) in the IPD (B, D) whereas wild type larva treated with 20 μM DAPT (A, D) does not. (E) Counting the number of cells positive for insulin revealed that 17 larvae depleted of sept7b and treated with 20 μM DAPT show altogether 12 cells positive for insulin in the IPD. In 17 wild type larvae treated with 20 μM DAPT altogether only four cells positive for insulin are observed in the IPD. In all cases, we observed only 0-2 cells/larva positive for insulin. Error bars represent the standard error of mean. * p≤ 0.05. Scale bar: A-D (25 μm).

Acknowledgments

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