ZFIN Image: Dash et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Pancreatic progenitors are increased in sept7b knockdown larvae.

(A?D) Pdx1-positive cells (red; arrow) in control MO-injected (A,C) and sept7b TBMO-injected (B,D) Tg(ptf1a:GFP) zebrafish larvae at 3?dpf. In (A,B) the exocrine pancreas (arrowhead) is visualized by ptf1a (green) and the nuclei are labelled with DAPI (blue). (C) and (D) are corresponding images visualizing Pdx1 only. (E) Pdx1-positive cells are significantly increased in sept7b knockdown larvae compared to control MO-injected larvae. (F) Schematic representation showing that control MO-injected larva show differentiation of Pdx1-positive pre-pancreatic progenitors, which give rise to mature insulin- and glucagon-positive ?- and ?-cells, respectively. The islet cells in sept7b TBMO-injected larva fail to differentiate and present bihormonal cells positive for both insulin and glucagon (see also Fig. 3). Scale bar: (A?D) (25??m). Error bars represent mean?�?SEM. *p???0.05.

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Acknowledgments

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