ZFIN Image: Dash et al., 2016, Fig. 2

Image

Figure Caption

Fig. 2

Knockdown of sept7b reduces β-cell volume and inhibits β-cell proliferation.

(A,B) Three-dimensional rendered z-stack confocal images generated from control MO-injected (A) and sept7b TBMO-injected (B) zebrafish larva at 5 dpf stained as whole mounts with antibodies against insulin. (C) Insulin-positive cell volume is significantly reduced in sept7b TBMO-injected zebrafish larvae at 5 dpf. (D,E) Three-dimensional rendered z-stack confocal images generated from control MO-injected (D) and sept7b TBMO-injected (E) zebrafish larva at 5 dpf treated with EdU (green) to visualize proliferating cells and stained with antibodies against insulin (red) to visualize β-cells. DAPI shows the nuclei (blue). Arrowheads indicate proliferating β-cells (positive for both EdU and insulin). (F) β-cell proliferation is significantly reduced in sept7b knockdown larvae. Error bars represent mean ± SEM. Scale bar (A,B) (10 μm); (D,E) (10 μm). ***p ≤ 0.0005.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.