ZFIN Image: Lamont et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Islet2a is negatively regulated by Notch signaling. (A–C) isl2a expression is strongly upregulated in rbpsuh morphants (B) as compared to control embryos (A). (C) Relative expression levels of isl2a and flt4 was increased by 2.5-fold and 2.3-fold, respectively, in rbpsuh morphants. (D–H) Representative photomicrographs showing the number of nuclei per ISA at 30 hpf in (D) wild type embryos, (E) Isl2^{ATG MO}, (F) Rbpsuh ^MO, (G) Rbpsuh ^MO with Isl2^{ATG MO} Lo (5.1 ng), (H) Rbpsuh ^MO with Isl2^{ATG MO} Hi (12.1 ng). (I) Quantitation of the average number of cells per ISA in single and double morphants. ** refers to p<0.005, and *** refers to p<0.0001 by an unpaired student's t-test. Scale bars represent 100 µm in A, B and 50 µm in D–H.

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Acknowledgments

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Reprinted from Developmental Biology, 414, Lamont, R.E., Wu, C.Y., Ryu, J.R., Vu, W., Davari, P., Sobering, R.E., Kennedy, R.M., Munsie, N.M., Childs, S.J., The LIM-homeodomain transcription factor Islet2a promotes angioblast migration, 181-92, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.