Fig. 1
Nrg2a is required for cardiac trabeculation in zebrafish.
(a,b) Zebrafish larvae from nrg2a+/? incrosses were imaged at 120?hpf; lateral view, anterior to the left; scale bars, 0.5?mm. nrg2a?/? larvae can be recognized by their defective jaws (asterisk in b). (c?k) nrg2a mutants lack cardiac trabeculae. Confocal images (mid-sagittal sections) of larval hearts from Tg(myl7:LIFEACT-GFP);nrg2a+/? incrosses at 75 (c?e), 120 (f?h) and 168?hpf (i?k); ventricular outer curvature (dashed boxes) magnified in upper right corners; arrows and asterisks indicate trabeculated and non-trabeculated walls, respectively; scale bars, 50??m.