Fig. 10

Fig. 10

Human AHI1 RNA rescues ahi1^lri46 mutant phenotype. (A–D) Whole-mount immunolabeling of pronephric cilia with anti-acetylated tubulin (green) at 36 hpf. Cilia are seen in pronephric ducts of wild-type animals and in ahi1^lri46 mutants following injection of mRNA encoding human AHI1 ([A, C]; arrows). Motor neurons also stained with acetylated tubulin ([B], arrowheads). (E) Quantification of cilia numbers in the distal pronephric duct of ahi1^lri46 mutants following injection. Distal pronephric cilia in wild-type animals were too numerous to count and are not presented graphically. (F–I) Images of 5 dpf retinas stained with PNA (red) and DAPI (blue) to label cone outer segments. (J) Quantification of cone outer segment lengths (n = 6). Scale bar: 20 μm.