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Fig. 1
Generation of a zar1 mutant with TALENs in zebrafish. (A) In situ hybridization on cryosections of wild-type gonads with zar1 antisense probe and sense probe. Scale bar: 200 μm. (B) Immunoblotting to detect Zar1 protein in stage I, stage II and stage III oocytes. Ten oocytes were lysed for each stage. (C) The TALEN sequences for zar1 mutant generation. (D) Digestion of PCR products from wild-type (WT) and zar1 TALEN mRNA injected embryos (TLN) with NdeI restriction enzyme. (E) DNA sequences of the zar1gd5 mutant fish line. A premature stop codon was generated. (F) Western blot of Zar1 in gonads from WT, heterozygotes (hetero) and homozygotes (homo) at 25 dpf. DNA sequences highlighted in yellow are the start codon; red, NdeI recognition sites; green, premature stop codon.