ZFIN Image: Miranda-Rodríguez et al., 2017, Fig. 4

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Fig. 4

mRNAs of vasa and nanos are mislocalized when RhoA is inhibited with C3. nanos mRNA in situ hybridization (A and B) and corresponding magnification insets (a and b). vasa mRNA in situ hybridization (C and D) and corresponding magnification insets (c and d). Embryos were injected Buffer (A, C, a and c) or with C3 (B, D, b and d) arrows indicate that there is no signal at the end of the cleavage furrow. Asterisk shows the characteristic localization of vasa mRNA at the periphery of the blastodisc present in control embryos (C) and absent in C3 injected embryos (D). (E) Normal localization of nanos of control embryos (n=94) and C3 injected embryos (n=99) were quantified and statistical difference was observed (left panel; 4 independent experiments). Normal localization of vasa observed in control embryos (n=98) and C3 injected embryos (n=113) was quantified (right panel; 5 independent experiments). The error bars represent the standard error of the mean (** p< 0.01 ***p<0.001).

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Acknowledgments

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Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.