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Fig. 7

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ZDB-IMAGE-161208-10
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Figures for Topczewska et al., 2016
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Fig. 7

RNAscope in situ hybridization and IHC for GFP performed for adults at 12 wpf.

(A-F) Sequential sections (4 μm) of interfrontal suture expression. Names of genes tested by RNAscope in situ hybridization are labeled on each image. (A, B) Representative images of the skull taken at low magnification (5x) that visualize entire expression pattern of (A) col1a1a and (B) col10a1a. (C-F) Images of interfrontal sutures and the expression of representative genes as labeled on each image. (G) Immunohistochemistry for sp7 observed in the interfrontal suture using a GFP reporter in the Tg(sp7:EGFP)b1212 transgenic line. Transverse paraffin section, GFP positive cells (green) and nuclei stained with DAPI (blue).

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