ZFIN Image: Cayuso et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Mosaic inactivation of EphrinB1 by Crispr/Cas9 editing, leads to defects in hepatoblast positioning and indicate long-distance contacts. Related to Figure 7.

(A-B') at 32 hpf, EphrinB1⁺-hepatoblasts transiently expressing lyn-Tomato distribute randomly throughout the bud (A,A’), while EphrinB1⁺ hepatoblasts (blue) aggregate mostly anterior and away from the right LPM and hepatoblasts lacking EphrinB1 form an elongated Prox1 domain (B,B’) closer to the midline (yellow arrowhead). White brackets indicate the length of the Prox1-domain. Confocal projections of ventral views; anterior to the top. (C-D') EphrinB1⁺ hepatoblasts (white) aggregate mostly away from the right LPM, often with a gap of 1-6 hepatoblasts lacking EphrinB1 (white arrowheads) indicating long-distance cell-cell contacts sensing repulsive EphB3b from the LPM. Consistently, hepatoblast protrusions (arrow) are also EphrinB1⁺ (D'). Transverse sections with dorsal to the top, and left to the right.

Acknowledgments

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Reprinted from Developmental Cell, 39, Cayuso, J., Dzementsei, A., Fischer, J.C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G.J., Ober, E.A., EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality, 316-328, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell