ZFIN Image: Shin et al., 2016, Fig. 4

Image

Figure Caption

Fig. 4

ERK signaling is essential for trunk lymphatic morphogenesis and differentiation. (A-I) Confocal images; lateral views, anterior to the left, dorsal is up. (A,B) pERK (red) and GFP (green) immunostaining in Tg(fli1a:egfp)^y1 embryos of the indicated genotype at 34 hpf. Arrows denote pERK-positive lympho-venous sprouts, arrowheads indicate pERK staining in motor neurons. Brackets indicate DA and PCV. (C) Prox1 (red) and pERK (green) immunostaining in a wild-type embryo at 38 hpf. Arrows denote endothelial cells that are positive for Prox1 and pERK in lymphatic sprouts or dorsal wall of PCV. (D-I) Embryos treated with DMSO (D-F) or 15 µM SL327 (G-I). Treatment time is indicated. (D,G) Tie2 immunostaining; arrows denote lympho-venous sprouts, PCV indicated by bracket. (E,H) Prox1 (red) and GFP (green) immunostaining of Tg(fli1a:egfp)^y1 embryos at 36 hpf; arrows denote Prox1-positive lymphatic sprouts, arrowheads indicate muscle pioneers and Corpuscle of Stannius (cs) is indicated. (F,I) Live Tg(fli1a:egfp)^y1 embryos at 49 hpf. Arrows indicate parachordal cells, or absence thereof.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development