ZFIN Image: Katz et al., 2016, Fig. 1

Image

Figure Caption

Fig. 1

Dynamic Expression of miR-9 in qNSCs

(A) qRT-PCR analysis of the seven miR-9 transcripts in 48 hr post-fertilization (hpf) embryos, 1 month post-fertilization (mpf) juvenile brains, and 3 mpf adult brains with the expression of each transcript over the sum expression of all transcripts.

(B) ISH against pri-miR-9-6 on a cross-section of the telencephalon illustrating its expression restricted to the ventricular region (blue). Scale bar, 100 μm.

(C) Sections through the medial region of the dorsal telencephalon showing ISH with a mature miR-9 LNA probe (magenta) and double fluorescent immunostaining for gfap:GFP (green) and MCM5 (light blue). miR-9 expression is restricted to qNSCs (gfap⁺, MCM5⁻; red arrows) and never observed in aNSCs (gfap⁺, MCM5⁺; white asterisk). Some qNSCs are devoid of miR-9 signal (yellow arrows). Scale bar, 10 μm.

(D) Proportion of qNSCs (gfap⁺, MCM5⁻; dark gray) and aNSCs (gfap⁺, MCM5⁻; light gray) in the total, miR-9⁺ or miR-9⁻ NSC population demonstrating that miR-9 expression is associated with quiescence.

(E) Percentage of qNSCs that are miR-9⁺ or miR-9⁻.

(F) Experimental design to assess miR-9 expression dynamics across the NSCs activation/quiescence cycle.

(G) Representative image of BrdU⁺ (light blue), miR-9⁺ (pink), and GS⁺ (green) NSC 10 days after BrdU pulse. Scale bar, 5 μm.

(H) Percentage of miR-9⁺ cells among BrdU⁺ NSCs after different chase period. ^∗p < 0.05; one-way ANOVA with Bonferroni post hoc correction. Data are represented as mean ± 95% confidence interval (CI); n = 3 brains per condition.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.