ZFIN Image: Cortes et al., 2016, Fig. S5

Image

Figure Caption

Fig. S5

Expression of Cxcl8 receptors cxcr1/cxcr2 in zebrafish HSPCs (related to Figure 5).

A. Expression analysis of FACS sorted populations showed enhanced cxcl8 expression following exposure to 1,25(OH)D3, with the biggest effect in Flk1⁺/cMyb⁺ HSPCs (cells sorted from ≥1000 embryos/condition x 2 replicates); error bars, mean ± SD.

B. qPCR analysis of ZFcxcr1 and Zfcxcr2 expression shows enrichment of ZFcxcr1 in FACS sorted HSPCs (Flk1⁺/cMyb⁺) and the Flk-/cMyb- population (n=2 replicates, cells sorted from ≥1000 embryos/condition); N.D. (not detected).

C. WISH analysis and phenotype distributions of embryos treated with 1,25(OH)D3 with and without the CXCL8 inhibitor SB25002 (1μM) showed co-treatment attenuated Vitamin D-mediated increases in runx1/cmyb expression (n≥50 embryos/condition).

D. FACS quantification of Flk1⁺/cMyb⁺ HSPCs after exposure to 1,25(OH)D3 with and without SB25002 (Con vs 1,25(OH)D3, *p<0.01; 1,25(OH)D3 vs SB25002, *p<0.01; Con vs 1,25(OH)D3 + SB25002, N.S.; (5 embryos/sample x 4 replicates/condition; error bars, mean ± SD ).

Scale bar 100μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.