ZFIN Image: Schulte et al., 2014, Fig. S4

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Figure Caption

Fig. S4

In vivo complementation of MEIS1, using a previously reported MO (meis1_MO2) against the acceptor splice junction of exon 2. (A) The effect of meis1_MO1 targeting the donor site of exon 2 and inducing skipping of exons 2-7 has been evaluated previously⁵. (B) We here amplified the cDNA sequence between exons 2-6 in control embryos and morphants injected with 9ng meis1_MO2 and detect only ~30% of the wild-type RNA message in the morphant embryos. (C-F) Suppression of meis1 results in a reduction of the size of the optic tectum by 20-30%, similar to the phenotype observed when using meis1_MO1 that can be significantly and reproducibly rescued upon co-injection with wt human MEIS1 mRNA. Asterisks denote significance levels as determined by Student`s t-test (** p-value < 0.005; * p-value < 0.05). MO=morpholino, wt=wildtype

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Hum. Genet.