Fig. 7
nr2f1a is required for the growth of ISV cells.
(A-D) TUNEL labeling and AO staining was used to detect apoptotic cells in wild type, and nr2f1ae2i2 morphants. Although some apoptotic cells were observed on the skin, cell death in vascular regions was not elevated compared to wild type controls. (E-H) The number of cells forming each ISV counted in wild type control Tg(kdrl:mCherry; fli1a:negfp)y7 (E) and nr2f1a morphant embryos (F) at 32 hpf. (G) Proportional distribution of ISVs containing 1-5 cells and (H) average ISV cells per ISV counted in both wild type and nr2f1a morphant. (*** refers to p<0.0001 by an unpaired student’s t-test).