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Fig. 2
Expression patterns of trpc1 in whole-mount zebrafish. A: Dorsal view of embryonic brain expression 24 hr postfertilization (hpf). B: Lateral close-up of trpc1 expression in CSG 24 hpf. C: Expression of trpc1 in the retinal neuroepithelium in higher magnification 48 hpf; anterior points to the top. D: Lateral view of a larva 3 days postfertilization (dpf) is shown with different focal planes in D and D2. E: Dorsal view of brain expression 3 dpf. F: Staining in the eye of larvae 3 dpf; anterior is up. G: Focus on expression in the habenulae 3 dpf in a dorsal view corresponding to the boxed region in E. Note the differential expression in the left (lHa) and right (rHa) habenula. H: Brain staining in a larva 5 dpf is shown in a lateral view. J.K: Dorsal views on larvae 5 dpf. More cells expressing trpc1 are present in the left compared with the right habenula. Anterior is to the left unless otherwise stated. CSG, cranial sensory ganglia; Die, diencephalon; GCL, ganglion cell layer; INL, inner nuclear layer; Tel, telencephalon; TeO, optic tectum; TG, trigeminal ganglia. Scale bars of 100 µm are indicated in the first picture of a series until changed. The scale bars = 50 µm in G,K.