Fig. 4

Fig. 4

znf644a and znf644b Morphant Retinas Are Composed of Distinct Populations of Retinal Cells with Differing Characteristics

(A) Immunostaining of retinal cross-sections monitoring BrdU incorporation and PCNA⁺ cells at 48 hpf in WT, znf644a, and znf644b morphants.

(B) Quantitation of BrdU⁺ cells/¼m² in central retina of WT, znf644a, or znf644b morphants at 48 hpf (n = 3 for each group). Error bars represent SD. p < 0.05, Student′s t test.

(C–E) Immunostaining of pH3 expression in retinal cross-sections at 48 and 72 hpf in WT, and znf644a morphants (C). Quantitation of pH3⁺ cells/¼m² in the central retina of WT, znf644a, or znf644b morphants at (D) 48 hpf (n = 5 for each group) and (E) 72 hpf (n = 2 for each group). Quantitation represented as mean ± SD.

(F) Immunostaining monitoring marker protein expression in retinal cross-sections. At 72 hpf, amacrine cells express GABA, bipolar neurons express PKC, and cone photoreceptors express Zpr1.

White arrows highlight populations of proliferative or differentiated cells.