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Fig. 2
Zebrafish znf644 Paralogs Are Specifically Expressed In and Regulate the Development of Retinal and Midbrain Progenitor Cells
(A) Conservation of C2H2-like ZF motifs between human and zebrafish ZNF644 genes.
(B) Representative lateral views, retinal cross-sections, and midbrain cross-sections of whole-mount in situ hybridization (WISH) assays monitoring znf644a or znf644b expression at 24 hpf (n > 15 in each group). Arrows denote the inner part of the central retinal epithelium.
(C) Lateral views of g9a (20/32), znf644a (10/18), or znf644b (14/21) morphant embryos compared with WT (21/21). Dashed circles denote 2D area of the WT retina. Arrowheads denote the dorsal hindbrain region.
(D) Quantitation (mean ± SD) of retinal 2D cross-sectional area (¼m2) of znf644a, znf644b, or g9a morphants relative to WT embryos. Error bars represent SD. *p < 0.005, Student′s t test.
(E) RT-PCR assays monitoring unspliced (unspl) and spliced (spl) znf644a or znf644b transcripts at 24 hpf.