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Fig. 5
Whole-mount antibody and in situ stainings of embryos for islet proteins (anti-pan-islet-antibody) (12 hour) and gsc (10 hour) visualizing the trigeminal placodes (islet) and prechordal plate (gsc) of wild type (A,B) and mbl (C,D) embryos. (A,C) Dorsal view of the head showing a reduction of gsc expression overlying the anteriorlateral edge of the prechordal plate (arrowheads) in mbl (anterior up). (B,D) Dorsal view of the head showing ectopic clusters of isletpositive cells (stars) anterior to the trigeminal placodes (arrowhead) in mbl (anterior to the left).
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